zagA [2019-05-29 17:06:31]

zinc metallochaperone, ZTP activated GTPase A

locus: BSU03360

pI: 4.40

mw: 45.15 kDa

protein length: 397 aa

gene length: 1194 bp

function: supply of zinc to [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|FolE]

product: ZTP activated GTPase A

essential: no

synonyms: yciC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.3|Zinc]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.4|Targets of ZTP]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 366,063 367,256

The protein

Protein family

• cobW C-terminal domain (according to Swiss-Prot)

Modification

• phosphorylated on Arg-58 [Pubmed|22517742]

Effectors of protein activity

• ZTP (stimulates interaction with [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|FolE]) [https://www.biorxiv.org/content/biorxiv/early/2019/03/31/594382.full.pdf reference]

Structure

• [PDB|1NIJ] (YjiA from E. coli, 27% identity) [pubmed|14696199]

[SW|Localization]

• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|F735802BB483C2C5437240AB2E9FC340EAA5B353|yczL]-[gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]
  description: [Pubmed|25649915]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12426338], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

• genes: [gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]-[gene|456AF184261976FAA73359E81443F7035F457D07|yciB]-[gene|F735802BB483C2C5437240AB2E9FC340EAA5B353|yczL]-[gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]
  description: [Pubmed|9811636]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12426338], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

  regulation

  • [SW|folE2]: induced by zinc starvation ([protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]) [Pubmed|12426338]

Biological materials

Mutant

• MGNA-B999 (yciC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1998 NBRP B. subtilis, Japan]
• BKE03360 ([gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAGTGCCTCCATTAA, downstream forward: _UP4_TAGAAAAAAAGCCGTCCCAT
• BKK03360 ([gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAGTGCCTCCATTAA, downstream forward: _UP4_TAGAAAAAAAGCCGTCCCAT

labs

• [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

• 12426338,9811636,18344368,22517742,18763711,23406412,27561249,14696199,31132310